LOCUS       40924_44819       13840 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pUB-GWS-GFP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, 
            Hayashi M.
  TITLE     Polyubiquitin Promoter-Based Binary Vectors for Overexpression and 
            Gene Silencing in Lotus japonicus
  JOURNAL   Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
  PUBMED    18321183
REFERENCE   2  (bases 1 to 13840)
  AUTHORS   Maekawa T, Hayashi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE   3  (bases 1 to 13840)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13840)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages: 
            "375-382"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2007) Contact:Makoto Hayashi National Institute of 
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(300..1016)
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(1067..1744)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    1935..1956
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1971..2001
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2009..2025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2033..2049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2071..2323)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2400..2524
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2549..2579
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2633..3289
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3634..3936
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3980..4104)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4138..4392
                     /note="contains intron 1 of Arabidopsis thaliana WRKY 
                     transcription factor 33"
     protein_bind    4447..4571
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4615..4917)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(5262..5918)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5972..6002)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(6027..6151)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6179..6195)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(6196..6782)
                     /gene="Ljubq1"
                     /label=Ljubq1
     intron          complement(6196..6735)
                     /gene="Ljubq1"
                     /note="5' UTR intron of Ljubq1 gene"
     exon            complement(6736..6782)
                     /gene="Ljubq1"
                     /number=1
     regulatory      complement(6783..7317)
                     /label=Ljubq1 promoter
                     /note="Ljubq1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(7344..7360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7563..7587
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8887..9513
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9950..11014
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      11083..11277
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    11621..11761
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11947..12535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12625..13416)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagccgggcg gccgctttac
      301 ttgtacagct cgtccatgcc gtgagtgatc ccggcggcgg tcacgaactc cagcaggacc
      361 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      421 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      481 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      541 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      601 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      661 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      721 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      781 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tgaaggtggt cacgagggtg
      841 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      901 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      961 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggga
     1021 tcgaattcct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
     1081 ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc
     1141 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     1201 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     1261 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     1321 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     1381 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     1441 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca
     1501 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     1561 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     1621 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     1681 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     1741 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     1801 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
     1861 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     1921 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     1981 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2041 agctatgacc atgattacga attcgagctc gatctagtaa catagatgac accgcgcgcg
     2101 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
     2161 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
     2221 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
     2281 gattcaatct taagaaactt tattgccaaa tgtttgaacg atcgatggcg cgcctaggta
     2341 ccggatccct gggtacctct cccggggatc ctctagtcta gaatcgatcg gaagttatca
     2401 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2461 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2521 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     2581 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     2641 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     2701 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     2761 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     2821 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     2881 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2941 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     3001 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     3061 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     3121 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     3181 gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag
     3241 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
     3301 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     3361 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag
     3421 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     3481 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     3541 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     3601 gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa
     3661 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     3721 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     3781 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     3841 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     3901 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     3961 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     4021 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     4081 ttcagctttc ttgtacaaag tggtgataac ccagtcccgg gcgatgaatt cctgtggttg
     4141 gagaagctag aacctgcaaa acaatcccaa gaattgttca gacttcaaga aaagagacaa
     4201 atagcaataa cataattaag aaagagtcga gagttgactc taaaagtctt tctctctctt
     4261 tgaaaccgtt gacccataac tttatcactt ccgatcacga ttcattgtca agatgttaaa
     4321 tcaatgatac atcaaacaca gattatcatc actaattgga ttcatttcaa aagaaacaga
     4381 gaaaaaaaac agagaggctt acgttagcag aggaggagaa gcttctagcc catggaacgg
     4441 aggatcacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     4501 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     4561 cagtcactat ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc
     4621 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
     4681 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct
     4741 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
     4801 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac
     4861 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
     4921 accagtccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
     4981 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
     5041 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
     5101 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcacacctct ttttgacata
     5161 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
     5221 tactgttatc tggcttttag taagccggat ccacgcgttt acgccccgcc ctgccactca
     5281 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca
     5341 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     5401 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     5461 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     5521 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     5581 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     5641 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     5701 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     5761 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     5821 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     5881 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     5941 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
     6001 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
     6061 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     6121 tttctcgttc agcttttttg tacaaacttg tgatcctcag gtggatcctc tttctcgaga
     6181 tccactagtt ctagactgta atcacatcaa caacagataa atcagaatcc aacacacgaa
     6241 attgaaacaa gtgaaaaccc taattaacgc ttggatcaaa cagattaaga gtggatctaa
     6301 catcaacaga taaatcagaa tccataacaa gaaattgaaa caagtgaaaa ccctaattga
     6361 agcttggatc aaacagatta agagtggatc taacaataat ttccaatttg aataaattat
     6421 ttgacataat tccataacgg atctgaagaa ttcaattgga aaccctaatt aatctgcaat
     6481 tgctaaaccg aaatcgattc ctaacaaaac tgaaccacaa ttatcataga tctacatcgc
     6541 aagccatcga aaacattgat aaaacaagat ctgagattca agctgtaaaa ttcaatttca
     6601 aaccgatctc aattttggaa aatcacaaaa aattcctaga tctcatgctt cgaaacaaga
     6661 aaactaccta tgattgatcg gaaacaaaac tcaacgaaag gattacagag aaagaagaga
     6721 gaaattggat cataccttga aagtagagag aagcgatatg aacaagaatt gttagggttg
     6781 ggaactgaat tgtgaagaag ctaaggggat tggaggtaat ttatagcaaa agcggtgaaa
     6841 ttattaacca gtcaaattgt gacacgtgtc cttatgacgc gtcactagtt gcgtgtggtt
     6901 tgggatagga tacgttgtgt ggttgagttc ttcttggaaa cggtagttgc aaattgcaag
     6961 ttgttggcgg tttgttaatt tcacggagtt aaacgcatct cgccgccttt tgagtttgga
     7021 ttgataaatc gttacaacgg cgttactttg ctgggccccg ttttttttga attgggccac
     7081 cgcaccagtg caagttgggg gttccttggt tttccagaat gggctccact ctctatgatt
     7141 gattggagga ggccggctgc tcagaaattt tataataagt tgttatatac ttctcccctt
     7201 aaaataagtg atttttgtct atccatagtt gcaataagtt gtactaacaa cttatctaaa
     7261 ttactttatc aataatacaa gtaattcaat taattatttc ctcaaaatcc tctctccatc
     7321 gaattcctgc agcccagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7381 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7441 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc
     7501 tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg
     7561 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga
     7621 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     7681 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     7741 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     7801 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     7861 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     7921 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     7981 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     8041 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     8101 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     8161 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     8221 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     8281 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     8341 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     8401 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     8461 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     8521 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     8581 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     8641 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     8701 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     8761 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     8821 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     8881 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     8941 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     9001 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     9061 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     9121 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     9181 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     9241 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     9301 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     9361 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     9421 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     9481 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     9541 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     9601 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     9661 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     9721 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     9781 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     9841 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     9901 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc
     9961 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
    10021 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
    10081 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
    10141 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
    10201 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
    10261 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
    10321 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
    10381 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
    10441 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
    10501 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
    10561 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
    10621 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
    10681 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
    10741 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
    10801 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
    10861 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
    10921 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
    10981 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
    11041 aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat
    11101 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
    11161 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
    11221 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
    11281 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
    11341 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
    11401 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
    11461 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
    11521 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    11581 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
    11641 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
    11701 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11761 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11821 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11881 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    12001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    12061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12121 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12361 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    12421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12481 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12541 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12601 tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt
    12661 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
    12721 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc
    12781 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc
    12841 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc
    12901 atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc
    12961 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt
    13021 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc
    13081 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc
    13141 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac
    13201 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt
    13261 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt
    13321 atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag
    13381 ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta
    13441 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
    13501 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
    13561 gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg
    13621 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
    13681 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
    13741 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
    13801 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
